Cloning and Prokaryotic Expression of the F Gene of NDV La Sota Strain

A pair of primers was designed according to the sequence of F gene of NDV La Sotastrain(AF077761) for amplifying the F gene by RT-PCR.1 700 bp DNA fragment was amplified and cloned into pGEM-T easy vector.After restriction endonuclease analysis and sequencing,the fragment was inserted into the expression vector pET-32a.The recombinant plasmid produced a 83 kd fusion protein in E.coli BL21(DE3) under the induction of IPTG.The expression quantity and immunoreactivity of the fusion protein was detected by SDS-PAGE electrophoresis and Western blotting.