2001•Zhongguo yufang shouyi xuebaoRequires access
Construction of the Recombinant Plasmid Coexpressing NDV F Gene and Chicken IL-2
Yonghou Jiang, Jiangli Chen, Song Xiulong, Tong Guang-zhi, Xiangang Kong, Liu Zhonggui
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Abstract
In this study,the NDV D26 strain F gene with CMV promotor and BGH polyA signal sequence was amplified by PCR from eukaryotic plasmid pcDNA_F,which contains the full_length NDV F gene,and cloned into reconstructed eukaryotic plasmid pcDNA_IL2,which contains chicken IL_2 gene.Restriction endonuclease cleavage and PCR amplification showed that a bicistronic plasmid encoding NDV F gene and chicken IL_2 separately was successfully constructed.