ScholarLens Back to search
2004•Zhongguo sheng-hua yaowu zazhiRequires access

Study on high density fermentation of recombinant Pichia pastoris expressing recombinant humace soluble TNF-related apoptosis-inducing ligand

Jidong Li

Open publisher page 0 citations

Abstract

PurposeTo study influential factors of the high density fermentation of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) Pichia pastoris.MethodsThe effects of different methanol inducement period, methanol concentration,pH were observed using shaking flask, and fed-batch fermentor was also studied.Results72.8 mg/L rhsTRAIL was obtained at pH 6.0,1.0% methanol on the fourth day and 184 mg/L rhsTRAIL were obtained in fed-batch fermentor.ConclusionThe developed fermentative technology might incease the expression of sTRAIL in recombinant Pichia pastoris strain.

About this research paper

What this paper is about

PurposeTo study influential factors of the high density fermentation of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) Pichia pastoris.MethodsThe effects of different methanol inducement period, methanol concentration,pH were observed using shaking flask, and fed-batch fermentor was also studied.Results72.8 mg/L rhsTRAIL was obtained at pH 6.0,1.0% methanol on the fourth day and 184 mg/L rhsTRAIL were obtained in fed-batch fermentor.ConclusionThe developed fermentative technology might incease the expression of sTRAIL in recombinant Pichia pastoris strain.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

PurposeTo study influential factors of the high density fermentation of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) Pichia pastoris.MethodsThe effects of different methanol inducement period, methanol concentration,pH were observed using shaking flask, and fed-batch fermentor was also studied.Results72.8 mg/L rhsTRAIL was obtained at pH 6.0,1.0% methanol on the fourth day and 184 mg/L rhsTRAIL were obtained in fed-batch fermentor.ConclusionThe developed fermentative technology might incease the expression of sTRAIL in recombinant Pichia pastoris strain.

Key concepts: Pichia pastoris, Recombinant DNA, Fermentation, Methanol, Pichia, Apoptosis, Chemistry, Tumor necrosis factor alpha

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on high density fermentation of recombinant Pichia pastoris expressing recombinant humace soluble TNF-related apoptosis-inducing ligand — Research Paper | ScholarLens