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2003•Chinese Journal of PharmaceuticalsRequires access

Effects of Fermentation Conditions on rhBSP by Recombinant Pichia pastoris

Dong Yan

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Abstract

Recombinant Pichia pastoris strain can express and secrete recombinant human bone sialoprotein (rhBSP). The optimum fermentation conditions of rhBSP were studied. Cells were harvested at A 600 =9.0 and resuspended in BMMY to an A 600 =45. rhBSP expression reached the largest amount 0.225 g/L at pH 6.0, 2.0% ( v/v ) methanol on the 4 th day.

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What this paper is about

Recombinant Pichia pastoris strain can express and secrete recombinant human bone sialoprotein (rhBSP). The optimum fermentation conditions of rhBSP were studied. Cells were harvested at A 600 =9.0 and resuspended in BMMY to an A 600 =45. rhBSP expression reached the largest amount 0.225 g/L at pH 6.0, 2.0% ( v/v ) methanol on the 4 th day.

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Available abstract

Recombinant Pichia pastoris strain can express and secrete recombinant human bone sialoprotein (rhBSP). The optimum fermentation conditions of rhBSP were studied. Cells were harvested at A 600 =9.0 and resuspended in BMMY to an A 600 =45. rhBSP expression reached the largest amount 0.225 g/L at pH 6.0, 2.0% ( v/v ) methanol on the 4 th day.

Key concepts: Pichia pastoris, Recombinant DNA, Chemistry, Fermentation, Pichia, Strain (injury), Methanol, Secretion

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