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THE VIABLE BUT NONCULTURABLE STATE OF Vibrio parahaemolyticus 1211U

Kou Yun

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Abstract

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50 60 days later, plate count(PC) and most probable number(MPN) showed that there were no culturable bacteria. But acridine orange direct count(AODC) showed that the total number of bacteria was not changed appreciably.Direct viable count(DVC) also confirmed that the number of viable Vibrio parahaemolyticus in the water was 105cells/ml.So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions.

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What this paper is about

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50 60 days later, plate count(PC) and most probable number(MPN) showed that there were no culturable bacteria. But acridine orange direct count(AODC) showed that the total number of bacteria was not changed appreciably.Direct viable count(DVC) also confirmed that the number of viable Vibrio parahaemolyticus in the water was 105cells/ml.So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions.

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Available abstract

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50 60 days later, plate count(PC) and most probable number(MPN) showed that there were no culturable bacteria. But acridine orange direct count(AODC) showed that the total number of bacteria was not changed appreciably.Direct viable count(DVC) also confirmed that the number of viable Vibrio parahaemolyticus in the water was 105cells/ml.So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions.

Key concepts: Vibrio parahaemolyticus, Viable but nonculturable, Microbiology, Plate count, Biology, Most probable number, Bacteria, Viable count

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