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2012•Chinese Journal of Tropical AgricultureRequires access

ISSR Analysis of 78 Mango Germplasm Genetic Relationships

Ying Dongshan

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Abstract

ISSR marker was used to analyze genetic diversities among 78 mango germplasm.12 primers selected from 100 ISSR-primers were used for amplification and a total of 148 DNA bands were obtained,including 142 polymorphic bands.The percent of polymorphic is 95.95%.The result showed that the cluster analysis based on ISSR data classified the mango germplasm into 5 major groups.In these groups,most of the samples(87.34%) were gathered in groupⅠ,indicating most of the mango germplasm with close genetic relationship.In this study,all cultivars tested were distinguished from each other and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mangoes.

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ISSR marker was used to analyze genetic diversities among 78 mango germplasm.12 primers selected from 100 ISSR-primers were used for amplification and a total of 148 DNA bands were obtained,including 142 polymorphic bands.The percent of polymorphic is 95.95%.The result showed that the cluster analysis based on ISSR data classified the mango germplasm into 5 major groups.In these groups,most of the samples(87.34%) were gathered in groupⅠ,indicating most of the mango germplasm with close genetic relationship.In this study,all cultivars tested were distinguished from each other and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mangoes.

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Available abstract

ISSR marker was used to analyze genetic diversities among 78 mango germplasm.12 primers selected from 100 ISSR-primers were used for amplification and a total of 148 DNA bands were obtained,including 142 polymorphic bands.The percent of polymorphic is 95.95%.The result showed that the cluster analysis based on ISSR data classified the mango germplasm into 5 major groups.In these groups,most of the samples(87.34%) were gathered in groupⅠ,indicating most of the mango germplasm with close genetic relationship.In this study,all cultivars tested were distinguished from each other and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mangoes.

Key concepts: Germplasm, Genetic diversity, Biology, Cultivar, Genetic relationship, Microsatellite, Genetic marker, Horticulture

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