Impact of Guilu Erxian Glue on Serum Type I Collagen Amino-terminal Extension Peptide in Ovariectomized Rat Model of Osteoporosis

Objective To observe the effect of Guilu Erxian glue on ovariectomized rat model of osteoporosis.Methods Fifty SPF healthy female SD 3-month-old rats were randomly divided into sham operation group,model group,treatment group,western medicine control group and traditional Chinese medicine control group. The rats in the sham operation group underwent sham surgery,and the other four groups underwent bilateral oophorectomy. We started the treatments 4 weeks after surgery. The rats were given Guilu Erxian glue by gavage in the treatment group[at a dose of 10 g /( kg·d) ],Xianlinggubao in traditional Chinese medicine control group[at a dose of 10 g /( kg·d) ],estradiol valerate tablets in western medicine control group [at a dose of 0. 2 mg /( kg·d) ],respectively. The rats in the sham operation group and model group were given normal saline by gavage( 1 ml /100 g by weight). The bone density of rats was measured using dual-energy X-ray absorptiometry 12 weeks after administration,and the blood samples were collected after anesthesia. Type I collagen carboxy-terminal peptide( β-Crosslaps),the total extension of type I collagen amino terminal peptide( P1NP),and the contents of Ca,P in serum of rats were measured in each group after administration.Results Compared with model group,serum Ca / P,bone density and P1 NP ratio of animals increased significantly in the sham operation group,treatment group,traditional Chinese medicine control group and western medicine control group( P 0. 05),and the β-Crosslaps content of animals decreased significantly( P 0. 01). The serum P content of animals decreased significantly in the treatment group,traditional Chinese medicine control group and western medicine control group( P 0. 05) compared with model group( P 0. 05). The serum Ca content of animals was significantly higher in the treatment group in contrast with those in the Chinese medicine control group and western medicine control group( P 0. 01),and the β-Crosslaps content of animals decreased significantly in the western medicine control group( P 0. 01). The bone density of animals in the treatment group and western medicine control group was significantly lower than that in the sham operation group( P 0. 01). No significant difference was observed in the P1 NP content among three treatment groups( P 0. 05). Conclusion Guilu Erxian glue may play an important role in the intervention on ovariectomized rats with osteoporosis.