Effect of GubenHuoxueZhuanggu Granules on IGF-1 level and IL-1β level in serum of ovariectomized rats with Osteoporosis

Objective:To explore the molecular mechanism of traditional medicine GubenHuoxueZhuanggu Granules on rats with Osteoporosis via measuring the level of IGF-1 and IL-1β in serum.Methods:Sixty three months old female SD rats were randomly divided into six groups(n=10):sham operation group,model group,XianlingGubao Capsule group,Nylestriol group,low-dose group and high-dose group of the traditional medicine GubenHuoxueZhuanggu Granules.A part of fat near the bilateral was removed from the rats of the sham operation group,while the bilateral ovaries of the rats in other groups were removed.After one week,the rats of the sham operation group and the model group were given normal saline(10 ml/kg),the rats of the XianlingGubao Capsule group were given the capsules called XianlingGubao(0.469 g/kg),the rats of the Nylestriol group were given Nylestriol(10ml/kg),while the rats of the low-dose group and the high-dose group of the traditional medicine GubenHuoxueZhuanggu Granules were given the granules of low-dose(0.938g/kg)and high-dose(4.69g/kg)respectively.The method of giving medicine was intragastric administration.After 90 days with intragastric administration,blood was collected from their hearts and serum Was separated.The level of IGF-1 and IL-1β were measured via the method of ELISA.The upper parts of the right shins of rats were made into pathologic slices.The differences of shapes and cellular morphology between of the pathologic slices of different groups were found via HE Staining.Results:Model group compared with sham operation group showed the lower level of IGF-1,IL-1β increased levels,and there wasf a significant difference(P0.05).Each group compared with model group increased IGF-1,IL-1β reduced.Nylestriol group and low-dose group compared with model group showed non-significance difference(P0.05).XianlingGubao Capsule group and high-dose group compared with model group have significant difference(P0.05).Conclusion:The traditional medicine GubenHuoxueZhuanggu Granules is effective for treating Osteoporosis of ovariectomized rats.It can raise IGF-1 level and lower IL-1β level,so it can improve bone tissue microstructure.