Analysis of differential gene expression profiles in hepatic fibrosis tissue using cDNA microarray

To investigate the differentially expressed genes in hepatic fibrosis tissues using cDNA microarray and screen for fibrosis-related genes.Total RNA was extracted from normal human liver tissues and liver cirrhosis tissues.The mRNA was used to prepare probes.The mixed probes were hybridized to the cDNA microarray.After washing,the DNA chips were scanned by laster scanner.The acquired image was analyzed.Among the 10.000 target genes,99 genes were identified in liver cirrhosis tissues that were either over or under-expressed as compared to controls.45 genes were up-regulated and 54 down-regulated.9 new genes were found in the study.The results showed that the cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic fibrosis-related genes.