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2006•Chinese journal of integrated traditional and Western medicineRequires access

Analysis of differential gene expression profiles in hepatic cellular carcinoma using cDNA microarray

Jun Zhou

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Abstract

Objective:To investigate the differentially expressed genes in hepatic cellular carcinoma(HCC)using cDNA microarray and screen for HCC-related genes.Methods:Total RNA was extracted from normal human liver tissues and hepatic cellular carcinoma tissues.The mRNA was used to prepare probes. The mixed probes were hybridized to the cDNA microarray. After washing, the DNA chips were scanned by laser scanner, The acquired was analyzed.Results:Among the 9182 target genes, 102 genes were identified in HCC tissues that were either over or under-expressed as compared to controls.Forty two genes were up-regulated and 60 down-regulated.Twelve new genes were found in the study. Conclusion: The cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic cellular carcinoma-related genes.

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Objective:To investigate the differentially expressed genes in hepatic cellular carcinoma(HCC)using cDNA microarray and screen for HCC-related genes.Methods:Total RNA was extracted from normal human liver tissues and hepatic cellular carcinoma tissues.The mRNA was used to prepare probes. The mixed probes were hybridized to the cDNA microarray. After washing, the DNA chips were scanned by laser scanner, The acquired was analyzed.Results:Among the 9182 target genes, 102 genes were identified in HCC tissues that were either over or under-expressed as compared to controls.Forty two genes were up-regulated and 60 down-regulated.Twelve new genes were found in the study. Conclusion: The cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic cellular carcinoma-related genes.

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Available abstract

Objective:To investigate the differentially expressed genes in hepatic cellular carcinoma(HCC)using cDNA microarray and screen for HCC-related genes.Methods:Total RNA was extracted from normal human liver tissues and hepatic cellular carcinoma tissues.The mRNA was used to prepare probes. The mixed probes were hybridized to the cDNA microarray. After washing, the DNA chips were scanned by laser scanner, The acquired was analyzed.Results:Among the 9182 target genes, 102 genes were identified in HCC tissues that were either over or under-expressed as compared to controls.Forty two genes were up-regulated and 60 down-regulated.Twelve new genes were found in the study. Conclusion: The cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic cellular carcinoma-related genes.

Key concepts: Complementary DNA, Microarray, Gene, Biology, Gene expression, Molecular biology, DNA microarray, Microarray analysis techniques

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