High performance liquid chromatography determination of vertilmicin in plasma using sensitive fluorometric derivatization

AIM: To develop a high-performance liquid chromatographic method with fluorescence detection for the determination of vertilmicin in plasma. METHODS: Derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by C_(18) reversed-phase chromatography allowed the fluorimetric detection of vertilmicin. Optimal conditions for the derivatization of vertilmicin are described.The mobile phase consisted of a mixture acetonitrile∶water (95∶5). A fluorescence was set as λ_(ex) 263 nm, λ_(em) 315 nm. RESULTS: The linear range of this method for determination of vertilmicin was 0.02-45 mg·L~(-1), the limit of quantification was 0.02 mg·L~(-1). The relative average recovery rates in plasma was 99.84 %. CONCLUSION: The method is sensitive and reproductive and this method can be used to determine vertilmicin in plasma.