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A Study of Preliminary Purification and Scavenging Free Radical Activity of Polysaccharide of Toona sinensis Roem

Yaming Zhao

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Abstract

In this experiment,the polysaccharide was extracted and purified from Toona sinensis Roem.We measured and purified the number of the polysaccharide protein,made an anti-oxide experiment for water-soluble polysaccharide and the purified polysaccharide,and measured the effect of eliminate of free radical for the different density of polysaccharide.The result shows that the composition of the polysaccharide was Xyl,Ara,Rha,Gal,Glc,Gal-A,and Man,and proportion of mol:1.0 ∶2.8 ∶2.6 ∶2.6 ∶2.0 ∶7.6 ∶1.6.A result obtained from anti-oxide experiment shows that different density of water-soluble polysaccharide and purified polysaccharide can eliminate free radical·OH and anions.Furthermore,the effect of purified polysaccharide is beyond of water-soluble polysaccharide.The water-soluble polysaccharide can eliminate R and the purified polysaccharide can not eliminate it.

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What this paper is about

In this experiment,the polysaccharide was extracted and purified from Toona sinensis Roem.We measured and purified the number of the polysaccharide protein,made an anti-oxide experiment for water-soluble polysaccharide and the purified polysaccharide,and measured the effect of eliminate of free radical for the different density of polysaccharide.The result shows that the composition of the polysaccharide was Xyl,Ara,Rha,Gal,Glc,Gal-A,and Man,and proportion of mol:1.0 ∶2.8 ∶2.6 ∶2.6 ∶2.0 ∶7.6 ∶1.6.A result obtained from anti-oxide experiment shows that different density of water-soluble polysaccharide and purified polysaccharide can eliminate free radical·OH and anions.Furthermore,the effect of purified polysaccharide is beyond of water-soluble polysaccharide.The water-soluble polysaccharide can eliminate R and the purified polysaccharide can not eliminate it.

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Available abstract

In this experiment,the polysaccharide was extracted and purified from Toona sinensis Roem.We measured and purified the number of the polysaccharide protein,made an anti-oxide experiment for water-soluble polysaccharide and the purified polysaccharide,and measured the effect of eliminate of free radical for the different density of polysaccharide.The result shows that the composition of the polysaccharide was Xyl,Ara,Rha,Gal,Glc,Gal-A,and Man,and proportion of mol:1.0 ∶2.8 ∶2.6 ∶2.6 ∶2.0 ∶7.6 ∶1.6.A result obtained from anti-oxide experiment shows that different density of water-soluble polysaccharide and purified polysaccharide can eliminate free radical·OH and anions.Furthermore,the effect of purified polysaccharide is beyond of water-soluble polysaccharide.The water-soluble polysaccharide can eliminate R and the purified polysaccharide can not eliminate it.

Key concepts: Polysaccharide, Chemistry, Chromatography, Biochemistry

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