2012•Zhongguo yaolixue tongbaoRequires access
Effect of Baixiangdan on the expression of 5-HTR2C and the concentration of IP_3 in neonate rat hippocampal neurons cultured in vitro
Yunxiang Su
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Abstract
Aim To explore the intervention mechanisms of baixiangdan capsule by observing the changes of the expression level of 5-HTR2C and the concentration of IP3 in neonate rat hippocampal neurons cultured in vitro.Methods Premenstrual syndrome(PMS) model rats with liver-qi invasion were induced by multiple emotional stimulations and baixiangdan capsule was administered after PMS induction.The medicated serum was used to neonate rat hippocampal neurons.The expression level of 5-HTR2C in the neurons was evaluated by Western blot and the concentration of IP3 was measured with the Elisa method.Results Compared with control group,both the protein expression of 5-HTR2C and the concentration of IP3 of PMS liver-qi invation group were significantly higher in neurons(P0.01,P0.01).After treated with baixiangdan medicated serum,the expression of 5-HTR2C and the concentration of IP3 in hippocampal neurons were significantly improved(P0.01,P0.01).Conclusions PMS model rats with liver-qi invasion serum can up-regulate the expression of 5-HTR2C in hippocampal neurons and increase the concentration of the second messenger IP3.Baixiangdan capsule might partially play its role in ameliorating PMS by regulating the expression of 5-HTR2C and changing the concentration of IP3.