Effect of Jingqianshu Granule on the Expression of 5HT_(1A) Receptor in Neonate Rat Hippocampal Neurons Cultured in vitro

Objective:To study the effect of Jingqianshu granule,a Chinese patent drug,on expression level of serotonin 1A receptor(5-HT1AR) and corpuscular viability of neonate rat hippocampal neurons cultured in vitro.Methods:Premenstrual syndrome(PMS) model rats with liver-qi depression were prepared by binding method,the Jingqianshu granule medicated serum were used to culture neonate rat hippocampal neurons.The viability of neural cells was assayed by MTT;the expression levels of 5-HT1AR in the neurons were evaluated by western blot and RT-PCR.Results:Jingqianshu granule medicated serum could markedly increase the viability of neonate rat hippocampal neurons,up-regulate the expression of 5-HT1AR in hippocampal neurons.Conclusion:Premenstrual syndrome(PMS) model rats serum could down-regulate the expression of 5-HT1AR in hippocampal neurons,Jingqianshu granule might partially in ameliorate premenstrual syndrome by increasing the viability of neonate rat hippocampal neurons and up-regulating the expression of 5-HT1AR in them,there is at least one important target site of 5-HT1AR for Jingqianshu granule in neonate rat hippocampal neurons.