2004•Chieh P'ou Hsueh PaoRequires access
BASIC BIOLOGICAL CHARACTERISTICS, ISOLATION AND PURIFICATION OF MESENCHYMAL STEM CELLS DERIVED FROM EMBRYONIC BONE MARROW
Hui Qian, Wenrong Xu, Xiran Zhang, Xiaochun Sun, Wei Zhu, Jiabo Hu
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Abstract
Objective To isolate mesenchymal stem cells (MSCs) from embryonic bone marrow and investigate their basic biological characteristics. Methods MSCs were separated from embryonic bone marrow by 1073 g/L Ficoll reagent and expanded in culture medium. The proliferation and growth characteristics were observed in primary and passage culture. The surface anigens and cell cycle of MSCs were analyzed by flow cytometer. The karyotype of MSCs was detected. Results After three days of culture, MSCs began to adhere to culture bottle and displayed small spindle shape in culture. After ten days, it began to confluence and appeared to be fibroblast-like. 1×10 12 ～5×10 12 cells were got approximately after passage 10. MSCs were positive for CD13, CD29, CD44, CD71 and negative for CD3,CD14, CD33, CD34, CD38, CD45, HLA-DR.The karyotype of MSCs was normal. G-0/G-1 phase: 846%, G-2/M phase: 299%, S phase: 1235%.Conclusion MSCs have strong self-renewal capacity and can be used as the seed cells for tissue engineering.