Cultivation and identification of human mesenchymal stem cells

Objective:To explore a practical method of separation and in vitro cultivation of mesenchymal stem cells (MSCs) from human bone marrow, and to offer carrier cell for gene therapy.Method:MSCs from human bone marrow were separated and purified by means of gradient centrifugation and adherence to the culture plastic, which were detected by Flow cytometry with cell surface markers. Result:MSCs had spindle shape and had a typical fibroblast-like morphology .MSCs expressed positive for CD90. The cells were noted to have a simple shape after subculture, which had a higher expression of CD90. 94.31% of the third generation cells showed positivity to CD90.Conclusion:MSCs were obtained from human bone marrow in vitro, which had a high purity with passage culture. This methods is suitable for MSCs.