Role of PI3K/Akt signaling pathway in P12 cells apoptosis induced by β-amyloid peptide

AIM: To investigate the role of PI3K/Akt signaling pathway on cell apoptosis induced by β-amyloid peptide in PC12 cells.METHODS: The viability of PC12 cells and the levels of p-AktSer473 and p-GSK-3βSer9 were detected by MTT and Western blotting,respectively.Cell apoptosis was determined by Annexin V-PI staining.RESULTS: Aβ25-35 treatment decreased the viability of PC12 cells in a time-depended manner(P0.05).Annexin V-PI staining demonstrated that Aβ25-35 induced PC12 cells apoptosis in a time-depended manner(P0.05).Western blotting showed that Aβ25-35 induced an immediately decreased expression of p-AktSer473 and p-GSK-3βSer9 at 30 min(P0.05),but increased at 3 h and decreased again at 6 h.However,after 12 h of peptide treatment,the p-GSK-3βSer9 increased while the p-AktSer473 remained decrease.CONCLUSION: PI3K/Akt signaling pathway is involved in cell apoptosis induced by β-amyloid peptide,which provides a new clue for the study of pathogenesis and management of AD.