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2010•Zhongliu fangzhi yanjiuOpen access

Effect of Integrin-mediated Signal Transduction Pathways on Apoptosis of K562 Cells

Ling Pan

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Abstract

Objective To evaluate the role of integrin mediated signal transduction pathway in the apoptosis of K562 cells.Methods The percentage of apoptotic K562 cells induced by Anti-α5β1 was measured by FCM and acridine orange/ethidium bromide (AO/EB) double fluorescent dye staining, the expression levels of PI3K and survivin mRNA were measured by RT-PCR. The AKT and PI3K protein expression levels and the phosphorylation levels of these proteins were detected by Western blot method.Results Anti-α5β1 antibodies induced apoptosis of K562 cells and down-regulated the expression levels of PI3K mRNA and protein, survivin mRNA and AKT protein. The effect of Anti-α5β1 was much stronger than that of IFNα-2b.Conclusion The PI3K-AKT-survivin signal transduction pathway mediated by integrin α5β1 might contribute to the apoptosis of K562 cells.

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Objective To evaluate the role of integrin mediated signal transduction pathway in the apoptosis of K562 cells.Methods The percentage of apoptotic K562 cells induced by Anti-α5β1 was measured by FCM and acridine orange/ethidium bromide (AO/EB) double fluorescent dye staining, the expression levels of PI3K and survivin mRNA were measured by RT-PCR. The AKT and PI3K protein expression levels and the phosphorylation levels of these proteins were detected by Western blot method.Results Anti-α5β1 antibodies induced apoptosis of K562 cells and down-regulated the expression levels of PI3K mRNA and protein, survivin mRNA and AKT protein. The effect of Anti-α5β1 was much stronger than that of IFNα-2b.Conclusion The PI3K-AKT-survivin signal transduction pathway mediated by integrin α5β1 might contribute to the apoptosis of K562 cells.

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Available abstract

Objective To evaluate the role of integrin mediated signal transduction pathway in the apoptosis of K562 cells.Methods The percentage of apoptotic K562 cells induced by Anti-α5β1 was measured by FCM and acridine orange/ethidium bromide (AO/EB) double fluorescent dye staining, the expression levels of PI3K and survivin mRNA were measured by RT-PCR. The AKT and PI3K protein expression levels and the phosphorylation levels of these proteins were detected by Western blot method.Results Anti-α5β1 antibodies induced apoptosis of K562 cells and down-regulated the expression levels of PI3K mRNA and protein, survivin mRNA and AKT protein. The effect of Anti-α5β1 was much stronger than that of IFNα-2b.Conclusion The PI3K-AKT-survivin signal transduction pathway mediated by integrin α5β1 might contribute to the apoptosis of K562 cells.

Key concepts: Survivin, PI3K/AKT/mTOR pathway, Apoptosis, Acridine orange, Signal transduction, Protein kinase B, K562 cells, Molecular biology

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