Determination of Organic Acid in Apple Vinegar by Reversed Phase High Performance Liquid Chromatography

Determination of organic acid in apple vinegar beverage and apple vinegar by reversed phase high performance liquid chromatography was studied.The separation was performed on a C18 column with 0.01 mol/L diammonium phosphate-phosphate buffer solution as mobile phase,the flow rate and detection wavelengths were set to be 1.0 mL/min and 210 nm,respectively.Experimental results showed that the maximum content of organic acid was obtained in the apple juice,and the sample recovery rate was in the range of 90.8%~99.5%.It was proved that the accuracy of this method was very high and it's completely applicable to test the contents of organic acid in apple vinegar.