Cloning of human B7-1 gene cDNA and preparation of adenovirus vector carrying B7-1

Objective: To construct adenovirus vector carrying human B7 1 gene for tumor gene therapy. Methods and Results: RT nest PCR was used to clone human B7 1 gene cDNA from mixed human bone marrow cell culture. Then a recombinant adenovirus vector was constructed, which carrying B7 1 or LacZ. Human B7 1 gene cDNA and adenovirus vector carrying B7 1 were identified by enzyme digestion and DNA sequencing. The infectious adenovirous carrying B7 1 gene were produced by 293 packaging cells co transfected by the recombinant plasmid and plasmid BHG10. The adenovirus were used to infect stamoch cancer cells SGC7901 in vitro . Cells infected by Ad LacZ were dyed blue by X gal while B7 1 gene was detected by PCR in cells infected by Ad B7 1. Conclusion: The recombinant adenovirus vector can efficiently transfer LacZ and B7 1 gene into tumor cells. This result suggests that it can be used in the future for tumor gene therapy. [