The construction and Identification of Recombinant Adenovirus Vector of hIL-24

Objective To construct and identify the recombinant adenovirus vector of human IL-24 gene for future gene therapy of pathological scar tissue. Methods The human IL-24 gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria of PAdEasy system. Then the right recombinant adenovirus was transfected into 293A cells using Lipofectine 2000. The recombinant adenovirus vector was identified by restriction enzyme digestion and polymerase chain reaction (PCR). Results Restriction endonuclease and PCR analysis confirmed that the human IL-24 gene was successfully inserted into the adenovirus vector. The titer of the recombinant adenovirus was 107pfu/ml. Conclusion The recombinant adenovirus vector of hIL-24 was successfully constructed with highly infection.