Effect of lipopolysaccharide binding protein gene polymorphism on lipopolysaccharide-induced LBP and inflammatory cytokine levels

Objective To evaluate whether the lipopolysaccharide binding protein (LBP) C1306→T single nucleotide polymorphism influences the LBP and inflammatory cytokine levels in whole blood culture. Methods In 118 healthy human blood donors, the C1306→T gene polymorphism was determined by using polymerase chain reaction and subsequent Stu I restriction enzyme digestion of the polymerase chain reaction products. Supernatant concentrations of LBP, TNF-α, IL-6 and IL-10 were measured by using a whole blood cell culture model in the presence or absence of lipopolysaccharide ( LPS) stimulation. Results Among the 118 individuals, 14 subjects were heterozygous and 104 homozygous for the T/T allele.The LBP levels in supernatant were higher in T/C heterozygotes than in the individuals homozygous for the T allele either before or after LPS stimulation, while no marked correlations were found between LBP C1306→T polymorphism and TNF-α, IL-6 or IL-10 formation to LPS (P 0.05).Conclusion The LBP C1306→T polymorphism may influence the LBP level indirectly, but did not significantly relate to inflammatory cytokine in vitro.