2010•Lara D. VeekenRequires access
Cytokines and Inflammatory Mediators [30-39]: 30. The LPS Stimulated Production of Interleukin-10 is not Associated with -819C/T and -592C/A Promoter Polymorphisms in Healthy Indian Subjects
Sandeep Kumar Shukla, Able Lawrence, Aakanksha Aggarwal, S. Naik, Nicola Gullick, Hayley G. Evans, D. Jayaraj, Bruce Kirkham, Leonie S. Taams, Sunita Maya Judah, Nancy Nixon, P. T. Dawes, Derek L. Mattey, L. Yeo, Caroline Schmutz, Kai‐Michael Toellner, M. Salmon, Andrew Filer, C. Buckley, K. Raza, Dagmar Scheel‐Toellner, Misato Hashizume, Hisahiro Yoshida, Nobuo Koike, Misa Suzuki, Makoto Mihara, Antonios Stavropoulos‐Kalinoglou, George S. Metsios, Karen Douglas, V. F. Panoulas, Yiannis Koutedakis, George D. Kitas, Leigh D Church, Andrew Filer, E. Hildago, Katherine Howlett, Andrew Thomas, Stephen Rapecki, Dagmar Scheel‐Toellner, C. D. Buckley, K. Raza, Maria Juárez, Jillian Kolasinski, Jyothish Govindan, A. Quilter, L. Williamson, D. A. Collins, Elizabeth Price, Armen Yuri Gasparyan, Antonios Stavropoulos‐Kalinoglou, Tracey E. Toms, Karen Douglas, George D. Kitas, Helen J. Lachmann, J. B. Kuemmerle‐Deschner, É. Hachulla, J. Hoyer, J. P. Smith, KS Leslie, Isabelle Koné‐Paut, Jan-Matthias Braun, Andrea Widmer, Niren Patel, R. Preiss, Philip N. Hawkins
Abstract
Background: Interleukin-10 is a pivotal immunoregulatory cytokine with pleiotropic effects on the immune system. IL-10 promoter polymorphisms have been associated with disease susceptibility and the ability to secrete IL-10 in vitro. We suspected that the association of the widely studied -819C/T and -592C/A polymorphisms with the IL-10 production might vary between ethnic groups. Therefore, we examined the association of -819 C/T and -592 C/A promoter polymorphisms with in vitro LPS stimulated secretion of IL-10 in normal healthy Indian volunteers. Methods: Peripheral blood was collected from 103 healthy volunteers and diluted whole blood cultures were set up with 100 ng/ml of LPS as stimulant: supernatant was collected at 24 h and IL-10 levels were assayed by ELISA. Genotyping was done for -819C/T polymorphism in 101 individuals and -592C/A polymorphism in 68 individuals by polymerase chain reaction followed by RFLP. The differences in IL-10 production between the genotypes were analysed by ANOVA. Results: There were 30, 47 and 24 individuals with the CC, CT and TT genotypes with a minor allele (T) frequency of 47% for the -819C/T polymorphism. The CC and TT genotypes at position -819 were strongly associated with CC and AA genotypes at -592 position suggestive of strong linkage disequilibrium. There was no association between the -819 genotype and the in vitro LPS stimulated IL-10 levels. Conclusions: The -819C/T and the -592 C/A polymorphisms of the IL-10 promoter region are not significantly associated with LPS stimulated IL-10 production healthy Indian subjects. Disclosure statement: All authors have declared no conflicts of interest.