Analysis of Phenolic Compounds and Their Antioxidant Activity in Fruits from Different Pear Cultivars
LI Li-me
Abstract
LI Li-me
Abstract
The contents and composition of soluble phenolic compounds in pear peel, core and flesh from 11 major cultivars in northern China were determined and analyzed by high performance liquid chromatography(HPLC). Meanwhile, the antioxidant activities of their ethanol extracts were evaluated by DPPH and hydroxyl scavenging radical assays. Results indicated that: 1) the highest contents of soluble phenolic compounds were found in the core, followed by the peel and flesh; 2) 8 kinds of phenolic compounds, i.e., arbutin, gallic acid, catechinic acid, chlorogenic acid, caffeic acid, epicatechin, cumaric acid and rutin were detected in both the core and peel, while 5 kinds of phenolic compounds, i.e., arbutin, gallic acid, chlorogenic acid, caffeic acid and epicatechin were detected in the flesh; 3) chlorogenic acid, arbutin and epicatechin were the major phenolic compounds in the peel and flesh with higher content of chlorogenic acid than arbutin in the flesh and the opposite result for the peel. At the same time, the main phenolic constituents of the core were chlorogenic acid and arbutin with higher level of the former when compared with the latter; 4) for each of the three fruit tissues, significant positive correlations between phenolic compound contents and DPPH and hydroxyl radical scavenging activities were observed, suggesting the close association of phenolic compounds with free radical scavenging activity.
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The contents and composition of soluble phenolic compounds in pear peel, core and flesh from 11 major cultivars in northern China were determined and analyzed by high performance liquid chromatography(HPLC). Meanwhile, the antioxidant activities of their ethanol extracts were evaluated by DPPH and hydroxyl scavenging radical assays. Results indicated that: 1) the highest contents of soluble phenolic compounds were found in the core, followed by the peel and flesh; 2) 8 kinds of phenolic compounds, i.e., arbutin, gallic acid, catechinic acid, chlorogenic acid, caffeic acid, epicatechin, cumaric acid and rutin were detected in both the core and peel, while 5 kinds of phenolic compounds, i.e., arbutin, gallic acid, chlorogenic acid, caffeic acid and epicatechin were detected in the flesh; 3) chlorogenic acid, arbutin and epicatechin were the major phenolic compounds in the peel and flesh with higher content of chlorogenic acid than arbutin in the flesh and the opposite result for the peel. At the same time, the main phenolic constituents of the core were chlorogenic acid and arbutin with higher level of the former when compared with the latter; 4) for each of the three fruit tissues, significant positive correlations between phenolic compound contents and DPPH and hydroxyl radical scavenging activities were observed, suggesting the close association of phenolic compounds with free radical scavenging activity.
Key concepts: Chlorogenic acid, Arbutin, Gallic acid, Chemistry, Caffeic acid, Flesh, DPPH, Rutin