Detection and Drug Resistance of Extended-spectrum β-lactamase-Producing Escherichia coli in Patients with Urinary Tract Infection

Objective To explore the drug resistance of Escherichia coli and the situation of producing extended-spectrum beta-lactamases(ESBLs) in patients with urinary tract infection,and to provide the reference for clinical medication.Methods The identification of Escherichia coli and its drug-sensitivity to 20 kinds of common antibiotics were tested by Sunshine Semi-automatic Microbial Analyzer and Disk Diffusion Testing(Kindy-Bauer Testing).Results Among 591 strains of Escherichia coli isolated from urine samples of inpatients with urinary tract infection from March 2008 to March 2011,327 extended-spectrum beta-lactamases-producing strains were detected,with the detection rate of 55.33%.The drug-sensitivity to 20 kinds of common antibiotics showed that the resistance rates of ESBLs-producing E.coli to penicillin antibiotics,compound sulfanomides,cephalosporin antibiotics,quinolone antibiotics,and aminoglycoside were more than 90%,84.71%,84.40%~98.78%,67.89%~68.81%,and 16.82%~60.86%,respectively.The resistance rates of non-ESBLs-producing E.coli to penicillin antibiotics,compound sulfanomides,cephalosporin antibiotics,quinolone antibiotics,and aminoglycoside were 39.77%~70.45%,64.02%,8.71%~37.88%,40.53%~42.05%,and 7.95%~45.45%,respectively.The resistance rates of ESBLs-producing E.coli to common antibiotics were significantly higher than those of non-ESBLs-producing E.coli except nitrofurantoin(P0.05).Conclusions Producing ESBLs is the main reason for antibiotic resistance of Escherichia coli isolated from urine samples of inpatients with urinary tract infection in Hunan Provincial People's Hospital.It is of great value to monitor bacterial resistance for rational use of antibiotics in clinical practice.