STUDY ON THE PROTECTIVE IMMUNITY ACTIVATED BY ANTIGENS OF ADULT WORMS,NEWBORN LARVAE AND MUSCLE LARVAE OF TRICHINELLA SPRIALIS

Aim To compare the protective immunity against challenge infection in mice immunized by crude antigen preparations derived from adult worms,newborn larvae and musele larvae of T richinella spiralis.Method Intestinal adult worms,muscle larvae and blood absolute eosinophil level were counted;the level of serum IgG to antigens of T.spiralis muscle larvae was detected by ELISA.Results Adults reduimg rate was 82.19%,72.31% and 42.88% in the adult,newborn larvae and muscle larvae antigen groups respectively.Muscle larvae were reduced 68.83%.78.19% and 51.96% respectively.The serum IgG titer in all immunized groups increased significantly,the GMRT values of adult,newborn larvae and mudscle larval antigen groups were 7.46,5.28and 4.92 times higher than that in control group respectively.Periphery blood absolute eosinophil level enhanced significantly.Conclusion The data demonstrate that all of the adult,newborn larvae and muscle larvae antigens of T.spiralis can activate specific humoral and cellular immunity which induce protection to challenge infection in mice.Among these antigens,adult and newborn larvae antigens show better immunogenicity and it may be possible that adult antigen will provide a potential vaccine for trichinosis.