Study on Neuroprotective effects of edaravone on substantia nigra in rat model of parkinson's disease

Objective:To investigate the edaravone neuroprotective effect on the 6-OHDA-induced rat model of Parkinson's disease and its mechanism. Methods: The experimental animals were divided into two groups including the neurobiochemistry group and the histolology group, each of which was divided into three subgroups: the normal control group(n=6), the normal saline(NS)group(n=6) and the edaravone group(n=24), and the edaravone group was again divided into four subgroups including the 0.3mg/Kg 14d group(n=6), the 1mg/Kg 14d group(n=6), the 3mg/Kg 14d group(n=6) and the 3mg/Kg 28d group(n=6). The model of Parkinson's disease was induced by injecting 6-OHDA into the left medial forebrain bundle. The effects of edaravone on the behavior of the rat and the number of positive cells of tyrosine hydroxylase(TH) and glutathione(GSH)in substantia nigra were observed. Results: Edaravone(3mg/kg14d,28d) dose-dependently decreased the rotation behaviors of rat models induced by apomorphine(P0.01),ameliorated the spontaneous behavior, and prevented the decrease of the number of SN TH-positive cells and GSH induced by 6-OHDA(P0.05). Conclusion: Edaravone had neuroprotective effects on the 6-OHDA-induced PD animal model, improved 6-OHDA injury in early rat behavioral change and inhibited depigmentation of DA neurons.