Effects of the ethanolic extract from Pinellia ternata on cell proliferation and cell cycle of human hepatoma HepG2 cell line

Aim:To investigate the effects of the ethanolic extract from Pinellia ternate(PTE) on HepG2 cells in vitro.Methods:The human hepatoma HepG2 cells were treated with different concentrations of PTE.The effect of different concentrations of PTE on cell growth of HepG2 was measured by MTT.Its effect on cell cycle distribution of HepG2 was detected by flow cytometry.And the effects on the expression of cyclin D1,c-myc and β-catenin were assayed by immunocytochemistry.Results:PTE significantly inhibited the growth of HepG2 cells after 24 h,48 h and 72 h treatment in a dose-dependent manner(P0.05).The inhibition rate reached 54.7% after being treated with 400 μg/mL of PTE.The cell proportion of HepG2 at G0/G1 phase in 400 μg/mL PTE-treated group was significantly up-regulated(66.5±2.1)% compared with that of the control(58.6±1.3)%(P0.01).Moreover,the expressions of cyclin D1,c-myc and β-catenin of PTE treated cells were significantly lower than that of the control(P0.05).Conclusion:PTE can induce the human hepatoma HepG2 cells arrested at G0/G1 phase,reduce the expression of cyclin D1,c-myc and β-catenin and inhibit cell proliferation.