ScholarLens Back to search
2013•Chinese Journal of Animal Infectious DiseasesRequires access

GENERATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CHICKEN INTERFERON β

Chan Ding

Open publisher page 0 citations

Abstract

Eight-week-old Balb/c mice were immunized with purified recombinant pCold-Ch IFN-β.Spleen cells were collected and fused with myeloma SP2/0 cells.Two hybridomas secreting antibodies against IFN-β were obtained and their ascitic ELISA titers were up to 103.Western blot analysis showed that both McAbs reacted with recombinant IFN-β protein.The minimum detection concentration of one McAb was below 15 pg/mL IFN-β.The generation of specific McAbs could provided an important tool for detection of IFN-β and evaluation of poultry vaccines.

About this research paper

What this paper is about

Eight-week-old Balb/c mice were immunized with purified recombinant pCold-Ch IFN-β.Spleen cells were collected and fused with myeloma SP2/0 cells.Two hybridomas secreting antibodies against IFN-β were obtained and their ascitic ELISA titers were up to 103.Western blot analysis showed that both McAbs reacted with recombinant IFN-β protein.The minimum detection concentration of one McAb was below 15 pg/mL IFN-β.The generation of specific McAbs could provided an important tool for detection of IFN-β and evaluation of poultry vaccines.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Eight-week-old Balb/c mice were immunized with purified recombinant pCold-Ch IFN-β.Spleen cells were collected and fused with myeloma SP2/0 cells.Two hybridomas secreting antibodies against IFN-β were obtained and their ascitic ELISA titers were up to 103.Western blot analysis showed that both McAbs reacted with recombinant IFN-β protein.The minimum detection concentration of one McAb was below 15 pg/mL IFN-β.The generation of specific McAbs could provided an important tool for detection of IFN-β and evaluation of poultry vaccines.

Key concepts: Recombinant DNA, Monoclonal antibody, Titer, Spleen, Western blot, Antibody, Virology, Interferon

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
GENERATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CHICKEN INTERFERON β — Research Paper | ScholarLens