2002•PubMedRequires access
[Preparation and application of monoclonal antibodies to recombinant human IFN alpha].
Meiying Wu, Yan-Ping Ai, Ren Ren, Yixin Liang, Jie Li, Wenguang Song, Dajun Yang, Jianbo Sun, Yong-qing Cheng
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Abstract
OBJECTIVE: To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha). METHODS: Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique. RESULTS: All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%. CONCLUSIONS: The highest purification efficiency was obtained by using 4G10 column.