Real-time Fluorescence Quantitative PCR in Detecting CD_(58) Gene Expression in PBMC in Patients with HBV Infection

Objective To quantitatively detect the expression level of CD_(58) in PBMC in the patients with HBV infection. Methods The total RNA isolated from PBMC was reversely transcribed into cDNA. The real-time fluorescence quantitative PCR method was used to analyze the expression level of CD_(58) gene in all patients and normal controls. The serum levels of ALT and AST were detected meanwhile. Results The real-time fluorescence quantitative PCR method was performed successfully to precisely detect the mRNA level. The expression level of CD_(58) gene in patients with HBV infection was significantly higher than those in control. The level of CD_(58) mRNA in PBMC from patients with HBV infection were positively correlated with serum levels of ALT and AST. Conclusion The real-time fluorescence quantitative PCR is the most precise method to quantitatively detect the mRNA level and can be used in gene expression changes. The expression level of CD_(58) gene is related with the degree of HBV infection and the degree of liver damage.