Inhibition of the cholangiocarcinoma cells by interferon- gamma to enhance the ability of tumor necrosis factor-related apoptosis inducing ligand

Objective To investigate the antitumor effects of tumor growth factor (TNF) related apoptosis inducing ligand (TRAIL) and the relative role of interferon gamma (IFN γ) on it in human cholangiocarcinoma cells.Methods Scanning and transmission electronic microscope,DNA gel electrophoresis,flow cytometric analysis were used to detect antitumor effects of TRAIL at various concentrations and study the effects of IFN γ on TRAIL in inhibiting human cholangiocarcinoma cells.Results After QBC 939 cells were treated with TRAIL at 100 μg/L for 24 h,the typical features of apoptosis were seen under transmission electronic microscopy and agarose gel electrophoresis of DNA extracted from QBC 939 cells.Flow cytometric analysis of QBC 939 cells demonstrated that the rate of apoptosis was (1.66± 0.73)%,(8.83±0.54)%,(22.30±0.64)%,(42.50±0.47)%,(49.06±0.72)% respectively after the cells incubated with TRAIL at different concentrations of 0,1,10,100,1?000 μg/L for 24 h. TRAIL could induce apoptosis of QBC 939 cells rapidly in a dose dependent manner as compared with control group ( P 0.01).IFN γ could enhance apoptosis induced by TRAIL.As the time or dose was increased the apoptosis was more significant ( P 0.01).Conclusion TRAIL could inhibit QBC 939 cell proliferation by inducing apoptosis.IFN γ could enhance apoptosis induced by TRAIL.