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Selection and study on characteristics of virus seed of HFRS Vero cell vaccine

Wei Wang

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Abstract

In preparation of virus seed of HFRS vero cells vaccine,PS-6(TypeⅠ) and L99(TypeⅡ)strains of HFRSVs were continuously propagated on the vero cells,and the both strains were examined on virus titers,immunogenicity and passage stability.Results showed that the two strains were adapted to the cell culture by continuous passages and the virus titers reached to 8.5lgCCID_(50)/ml.The neutralization antibodies reached to 1∶10,as determined by plaque reduction neutralization test(PRNT).The other results were in accordance with the requirements of regulations of biologics.Therefore the adapted PS-6 and L99 strains have now been applied to prepare HFRS vero cell vaccine.

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What this paper is about

In preparation of virus seed of HFRS vero cells vaccine,PS-6(TypeⅠ) and L99(TypeⅡ)strains of HFRSVs were continuously propagated on the vero cells,and the both strains were examined on virus titers,immunogenicity and passage stability.Results showed that the two strains were adapted to the cell culture by continuous passages and the virus titers reached to 8.5lgCCID_(50)/ml.The neutralization antibodies reached to 1∶10,as determined by plaque reduction neutralization test(PRNT).The other results were in accordance with the requirements of regulations of biologics.Therefore the adapted PS-6 and L99 strains have now been applied to prepare HFRS vero cell vaccine.

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Available abstract

In preparation of virus seed of HFRS vero cells vaccine,PS-6(TypeⅠ) and L99(TypeⅡ)strains of HFRSVs were continuously propagated on the vero cells,and the both strains were examined on virus titers,immunogenicity and passage stability.Results showed that the two strains were adapted to the cell culture by continuous passages and the virus titers reached to 8.5lgCCID_(50)/ml.The neutralization antibodies reached to 1∶10,as determined by plaque reduction neutralization test(PRNT).The other results were in accordance with the requirements of regulations of biologics.Therefore the adapted PS-6 and L99 strains have now been applied to prepare HFRS vero cell vaccine.

Key concepts: Vero cell, Immunogenicity, Virology, Titer, Plaque reduction neutralization test, Neutralization, Neutralizing antibody, Virus

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