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2004•Zhongguo meijie shengwuxue ji kongzhi zazhiRequires access

Dynamic Change of the Hantavirus Titers and the Amounts of Virus Antigen in Vero Cells

Zhu Zhi-yon

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Abstract

Objective To observe the adaptability of Hantavirus and the rule of these strains replication in Vero cells. Methods Virus are extracted every 3 days from Vero cells culture Hantavirus infected. Cytopathogenic effect (CPE) are observed until these cells degrade. Viral titers and the amounts of virus antigen after serial passages are observed by IFAT and RPHA. Results There was no cytopathogenic effect (CPE) in Vero cells after 32 days. Viral titers and the amounts of virus antigen reach to the top at 8- 11th day after Hantavirus infect Vero cells. Viral titers and the amounts of virus antigen still keep up at the level of 5.00 Lg TCID 50 /ml and 1∶20 at 32th day after infaction. Conclusion The results suggest that these 2 candidate strains had adapted to Vero cells possessed high titers and good antigenicity and be feasible to prepare the HFRS vaccine in Vero cells.

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Objective To observe the adaptability of Hantavirus and the rule of these strains replication in Vero cells. Methods Virus are extracted every 3 days from Vero cells culture Hantavirus infected. Cytopathogenic effect (CPE) are observed until these cells degrade. Viral titers and the amounts of virus antigen after serial passages are observed by IFAT and RPHA. Results There was no cytopathogenic effect (CPE) in Vero cells after 32 days. Viral titers and the amounts of virus antigen reach to the top at 8- 11th day after Hantavirus infect Vero cells. Viral titers and the amounts of virus antigen still keep up at the level of 5.00 Lg TCID 50 /ml and 1∶20 at 32th day after infaction. Conclusion The results suggest that these 2 candidate strains had adapted to Vero cells possessed high titers and good antigenicity and be feasible to prepare the HFRS vaccine in Vero cells.

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Available abstract

Objective To observe the adaptability of Hantavirus and the rule of these strains replication in Vero cells. Methods Virus are extracted every 3 days from Vero cells culture Hantavirus infected. Cytopathogenic effect (CPE) are observed until these cells degrade. Viral titers and the amounts of virus antigen after serial passages are observed by IFAT and RPHA. Results There was no cytopathogenic effect (CPE) in Vero cells after 32 days. Viral titers and the amounts of virus antigen reach to the top at 8- 11th day after Hantavirus infect Vero cells. Viral titers and the amounts of virus antigen still keep up at the level of 5.00 Lg TCID 50 /ml and 1∶20 at 32th day after infaction. Conclusion The results suggest that these 2 candidate strains had adapted to Vero cells possessed high titers and good antigenicity and be feasible to prepare the HFRS vaccine in Vero cells.

Key concepts: Vero cell, Virology, Antigenicity, Titer, Hantavirus, Biology, Virus, Antigen

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