The Effect of AG490 on the proliferation and apoptosis of human endometrial carcinoma cells

Objective To explore the effect of AG490 on the proliferation and apoptosis of human endometrial carcinoma cell line Ishikawa.Methods The cells after serum starvation were treated with AG490 with various concentrations (0, 25, 50, 100 μmol/L) for different durations (24, 48, 72 h). The effect of AG490 on cell proliferation was examined by MTT assay; the cell cloning efficiency was measured by plate clone formation assay; the morphology of the cells was observed by using immunofluorescence (IF) of Hoechst 33258 staining; the cell cycle and early apoptosis were detected by flow cytometry (FCM).Results AG490 inhibited the proliferation of Ishikawa cells in a time-and dose-dependent manner. AG490 significantly reduced the cloning efficiency of Ishikawa cells. The cell cycle was arrested in S phase by AG490 and AG490-induced apoptosis of Ishikawa cells in a time-and dose-dependent manner.Conclusion AG490 may be a new kind of treatment strategy for endometrial carcinoma.