The Study on Sterilization of Explant and Formation of Adventitious Bud for Cerasus subhirtella Miq. var. pendula Tanaka.

[Objective]The aim was to preliminaryly discuss sterilization of explant and formation of adventitious bud for Cerasus subhirtella Miq. var. pendula Tanaka. [Method]With the stems with buds of Cerasus subhirtella Miq. var. pendula Tanaka. as explants,the explant selection,methods and procedures of sterilization and adventitious bud induction were studied. [Result]The results showed that,the suitable explants acquisition time of midday,collectionsite was in the upper part of annual shoots; the procedures of surface sterilization was immersing 15 min of washing powder,water flushing 1 h,70% ethand soaking 30 s,0. 1% Hg Cl2 immerising 10 min,the sterile water rinsing 5 times,and reached the survival rate of 66%. The suitable inducing medium was 1 /2MS + 6-BA 0. 5 mg / L + NAA 0. 10 mg / L,and the differentiation rate reached 63. 6%. [Conclusion]The study provided related technical support for tissue culture of Cerasus subhirtella Miq. var. pendula Tanaka.