Magnetic resonance imaging and~1H magnetic resonance spectroscopy studies in rat brain C_6 glioma

Objective:To observe manifestations of magnetic resonance imaging (MRI) and 1H magnetic resonance spectroscopy ( 1H MRS) in rat brain C 6 glioma,correlative studies were performed between the manifestations of MRI and 1H MRS with histopathology.Methods:Rat C 6 glioma cells were cultured in vitro and prepared for intracerebral injection.cells were injected into the caudate nucleus of rat brain under guiding of stereotaxic apparatus to establish a rat brain glioma model.MRI and 1H MRS were conducted 12～18 days after implantation.The manifestations of MRI and 1H MRS were correlated with pathologic appearances.Results:16 brain glioma were developed in 24 rats after C 6 cell implantation.All rat brain C 6 gliomas presented as long T 1 and long T 2 signals on MRI and demonstrated obvious homogeneous or annular enhancement after the administration of gadolinium. 1H MRS showed that the NAA peak decreased or disappeared and NAA/Cr ratio decreased and Cho/Cr ratio increased obviously in C 6 glioma.Some of gliomas presented with Lac and/or Lip peak.Histopathologic analysis revealed that all the tumor growed invasively.As the volume of tumor increased,massive necrosis developed in the central area of tumor and the occurrence rates of Lac and/or Lip peak had increased in these lesions.Conclusion:Rat brain C 6 glioma is a very stable and ideal animal model for experimental glioma studies.MRI can provide its pathomorphologic information precisely and MRS can provide its metabolic and biochemical changes in vivo.