Molecular cloning and bioinformatic analysis of a novel pairing-associated gene SJCHGC00821 of Schistosoma Japonicum

Objective To clone Schistosoma Japonicum SJCHGC00821, a novel pairing-associated gene, and to construct its recombinant eukary-otic expression vector so as to explore its function and roles in pairing. Methods Schistosoma Japonicum SJCHGC00821 full length ORF was amplified by PCR from cDNA library of Schistosoma Japonicum. Recombinant eukaryotic expression vector(pcDNA3-SJCHGC) was then constructed by subcloning technique and confirmed by restriction enzyme digestion analysis and sequencing. Bioinformatic methods were used to analyze its possible structure and function. Results SJCHGC00821, with 597bp base pairs and coding for 198 amino acids, was amplified by PCR from cDNA library of Schistosoma Japonicum. The eukaryotic expression vector corresponding to SJCHGC00821 (pcDNA3-SJCHGC)was successfully constructed,which was confirmed by PCR and sequencing. The molecular weight is 22.76 kDa and the theoretical pI is 4.84. Secondary structure analysis showd that this protein had antigenicity. Conclusions Schistosoma Japonicum SJCHGC00821 gene has been successfully cloned, which will benefit further research on its function as well as its implications in pairing of Schistosoma Japonicum.