Molecular Cloning and Bioinformatic Analysis of a Novel Gene of SJFCE3549

Objective To clone Schistosoma japonicum SJFCE3549,a novel gene,to construct its recombinant eukaryofic expression vector,and to preliminarily explore its function and roles through bioinformatic methods.Methods The full-length ORF of Schistosoma japonicum SJFCE3549 was amplified by PCR from cDNA library of Schistosoma japonicum.Recombinant eukaryofic expression vector(pCMV-Tag2A-SJFCE3549) was then constructed by sub-cloning technique and confirmed by restriction enzyme digestion analysis and sequencing.Bioinformatic methods were used to analyze its possible structure and function.Results SJFCE3549,with 453bp base pairs and coding for 150 amino acids,was amplified by PCR from cDNA library of Schistosoma japonicum.The eukaryofic expression vector corresponding to SJFCE3549(pCMV-Tag2A-SJFCE3549) was successfully constructed,which was confirmed by PCR and sequencing.The molecular weight was 16.67 kDa and the theoretical pI was 6.28.Secondary structure analysis showed that this protein had antigenicity.Conclusions Schistosoma japonicum gene SJFCE3549 has been successfully cloned,which will benefit further research on its function.