The expression of TGF-β_1 in microglias in ischemic rat’s brain

Objective To clarify the role of TGF-β 1 in brain,and observe it’s expression in ischemic rat’s brain.Methods The64adult male healthy Wistar rats,weighting250-400g,were divided into8groups randomly (normal control,sham-operated,and groups by various postischemia moment being killed,from24h to336h,6/per group),then they were made model of FV-TFI (four-vessel model of transient forebrain ischemia),being observed TGF-β 1 ’s expression in ischemic rat’s brain on various postischemia moment from1to14days.immunohistochemical staining was performed in the whole brain tissue sections to determine where the TGF-β 1 was detected,and the protein levels it was expressed.Results In normal and sham-operated animals,a faint signal corresponding to TGF-β 1 immunoreactive protein was detected within all pyramidal hippocampal neurons,indicating that endogenous levels of this protein were low and were not induced by the surgical manipulations.The main regions where TGF-β 1 was detected were cortex and subcortical white matter,with many TGF-β 1 positive microglias,some pyknotic pyramidal neurons in cortex,a few macrophages was also noticed positive.The levels of TGF-β 1 in ischemic animals brains were in a developed mode,with a summit at1day returning to baseline after14days.Conclusion TGF-β 1 takes part in the whole brain ischemia and plaies an important role in ischemic injury,and it would provide a new therapeutic method to ischemic cerebrovascular disease.