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2014•China Medicine and PharmacyRequires access

Determination of Ferulic acid, and Caffeic acid in Rhizoma Chuanxiong. by HPLC

Jinxi Zhang

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Abstract

Objective To develop an HPLC method for Ferulic acid, Caffeic acid in Rhizoma Chuanxiong. Methods Kromasil-ODS(250mm×4.6mm, 5μm) column was used to separate the sample at the column temperature of 35 ℃. Methanol was used with a mobile phase of methanol –0.5% aetic acid(35:65, v/v) at a flow rate of 1.0mL/min. The detection wavelengths was set at 323 nm. Results The calibration curve was linear over the concentration of 13.62-257.4μg/mL for ferulic acid(r=0.9995). 29.40-515.7μg/mL for Caffeic acid(r=0.9996). The average recovery of Ferulic acid and Caffeic acid were 96.6%, 95.6%, and the RSD were 0.8%, 0.5%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.

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Objective To develop an HPLC method for Ferulic acid, Caffeic acid in Rhizoma Chuanxiong. Methods Kromasil-ODS(250mm×4.6mm, 5μm) column was used to separate the sample at the column temperature of 35 ℃. Methanol was used with a mobile phase of methanol –0.5% aetic acid(35:65, v/v) at a flow rate of 1.0mL/min. The detection wavelengths was set at 323 nm. Results The calibration curve was linear over the concentration of 13.62-257.4μg/mL for ferulic acid(r=0.9995). 29.40-515.7μg/mL for Caffeic acid(r=0.9996). The average recovery of Ferulic acid and Caffeic acid were 96.6%, 95.6%, and the RSD were 0.8%, 0.5%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.

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Available abstract

Objective To develop an HPLC method for Ferulic acid, Caffeic acid in Rhizoma Chuanxiong. Methods Kromasil-ODS(250mm×4.6mm, 5μm) column was used to separate the sample at the column temperature of 35 ℃. Methanol was used with a mobile phase of methanol –0.5% aetic acid(35:65, v/v) at a flow rate of 1.0mL/min. The detection wavelengths was set at 323 nm. Results The calibration curve was linear over the concentration of 13.62-257.4μg/mL for ferulic acid(r=0.9995). 29.40-515.7μg/mL for Caffeic acid(r=0.9996). The average recovery of Ferulic acid and Caffeic acid were 96.6%, 95.6%, and the RSD were 0.8%, 0.5%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.

Key concepts: Ferulic acid, Caffeic acid, Chromatography, High-performance liquid chromatography, Methanol, Medicine, Calibration curve, Chemistry

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