Relationship between PCNA expression and apoptosis of human lung cancer cell line

Objective To investigate the relationship between proliferating cell nuclear antigen (PCNA) expression and apoptosis of human lung cancer cell line.Methods Arsenic trioxide (As_2O_3) was used to induce the direct apoptosis in human lung cancer GLC-82 cell line. The MTT method and flowcytometry (FCM) were used for detection. With RT-PCR, the changes and relations of PCNA in the process of GLC-82 apoptosis were studied. Results The data showed that As_2O_3 significatly. inhibited proliferation of GLC-82 cells and inhibiting effects had a dose-and time-dependence. The change of DNA content of GLC-82 cell indicated that cell cycle progress could be blocked by As_2O_3 in G_2/M phase, with apperence of sub-G_1 peak in dose-dependent manner.And PCNA mRNA activation was inhibited by As_2O_3. Conclusion During apoptosis of GLC-82 cell induced by As_2O_3,PCNA activation could apparently be inhibited. Hence,inhibition of PCNA activation may represent a new chemosensitisation for tumors resistant to anticancer drugs.