Blood screening and amniotic fluid diagnosis of Down Syndrome in pregnant women at second rimester

Objective To analyze the effect of AFP andβ-HCG in serum of the pregnant women at the second trimester on the screening of foetus with Down syndrome.Methods The concentration of AFP andβ-HCG in serum were tested by microparticle enzyme.On the basis of some kinds of influencing factors,the risk assessment was made using analytical software.Down syndrome was finally diagnosed by analysis of chromosome in the cell of amniotic fluid.Results 3617 pregnant women at second trimester of pregnancy were screened and 219 were at high risk of Down syndrome with a positive rate of 6.1% .Test of chromosome in the amni- otic fluid cells of 178 high risk Down syndrome women was performed and chromosomal abnormality was observed in 6 of them and 3 were conformed to be with Down syndrome.Conclusion The analysis of AFP andβ-HCG in serum for diagnosis is safe,effective and simple for diagnosis of foetus with Down syndrome.