Inhibitory effect of anti-tumor componentIfrom Agkistrodon acutus venom on human lung cancer A549 cells

AIM:To research the inhibitory and apoptosis effect of anti-tumor component I(AAVC-I)from Agkistrodon acutus venom on human lung cancer A549cells.METHODS:The inhibition rate after 24hand 48hwith different concentrations of AAVC-I on A549was detected by MTT method.The form of apoptosis was observed by HE staining and Hoechst fluorescent staining.The expression change of bax protein after AAVC-I treatment was detected by immunohistochemical staining method. RESULTS:MTT showed that AAVC-I could inhibit the proliferation of A549in a time-and dose-dependent manner.The form of apoptosis in A549could be observed by ordinary microscope and fluorescent staining when treated with AAVC-I for 24h.Immunohistochemical staining showed that the mean light density had a trend of increase in dose dependency,which indicated that the expression of bax increased accordingly.CONCLUSION:AAVC-I had an inhibitory effect on A549cell proliferation,apoptosis promoting effect as well,which maybe related to the increase of bax expression.