Effects of Kaempferol on Apoptosis of Human Small Cell Lung Cancer H446 Cells

To investigate kaempferol induced apoptosis in small cell lung cancer cell lines H446 and its potential mechanism.MTT assay was used to determine the inhibitory rate of kaempferol with different concentrations on the proliferation of H446 cells.The cell apoptosis induced by kaempferol was detected with DAPI dye.RT-PCR and Western-blot were used to detect the changes of p53,bax and bc1-2 in mRNA and protein levels in H446 cells.MTT assay showed that the proliferation of H446 cells was markedly inhibited after treatment with kaempferol for 24 h;typical apoptotic morphology were observed in H446 cells with DAPI staining after induced by kaempferol.RT-PCR and Western-blot results indicated that the expression of p53 and bax were increased but bcl-2 was decreased by kaempferol.The result suggested that kaemperol could inhibit the proliferation of H446 cells and induce part of cells to apoptosis.p53,bax and bc1-2 may contribute to the apoptosis mechanism.