Determination content of compound vitamins A、E、K_1 injection by high performance liquid chromatography

Objective To determine the contents in compound vitamins A、E、K1 injection by high performance liquid chromatography(HPLC).Methods HPLC was used C18 column(150 mm×46 mm,5 μm)was selected,the mobile phase was methanol-alcohol(80∶20).Detection was performed by UV with wavelength of 270 nm.Results A linearity of retinyl palmitate was obtained from 125 to 125.5 μg/ml with good correlation(r=0.999 9).The average recovery of retinyl palmitate was 99.38%,and RSD Was 1.36%.A linearity of vitamin E was obtained from 125 to 1251 μg/ml with good correlation(r=0.999 8).The average recovery of vitamin E was 98.87%,and RSD was 0.95%.A linearity of vitamin K1 was obtained from 16.5 to 166.5 μg/ml with good correlation(r=0.999).The average recovery of vitamin K1 was 98.08%,and RSD was 1.57%.Conclusion The RP-HPLC is sensitive and accurate in determining the contents in compound vitamin injection.