The research on the expression and regulation of kallikrein 6 gene in human ovarian cancer cell

Objective To investigate the effects of estrogen on the expression of KLK6 mRNA and protein in human ovarian cancer cell line HO8910.Methods HO8910 cells were incubated for 72 hours in two groups:the test group with 17-βE_2 at different concentration(10~(-10)、10~(-9)、10~(-8)、10~(-7)mol/L)and the control group with Ethanol.Real-time fluorensence quantitive RT-PCR and flow cytometry were used to measured the expression of KLK6 mRNA and protein in the two groups.The cell proliferation was measured by 3-(4,5-dimethylthiazol-z-yl)-2,5-dipheny tetrazolium blue(MTT)calorimetric assay,while the cell cycle was determined by flow cytometry.Results The expression ofKLK6 mRNA(3.83±0.41、4.14± 0.49、6.26±0.38、7.28±1.82)and protein(10.62±0.35,10.89±0.12、11.88±0.28、12.07±0.15) in the test group of H08910 cells(10~(-10)10~(-9)10~(-8)10~(-7)mol/L)was higher than that in the ethanol control group(P0.01).MTT test showed the cell absorption value(0.771±0.015、0.849±0.022、 0.858±0.016、0.895±0.031)in the test group of HO8910 cells increased significantly,compared to that in the ethanol control group(0.459±0.064),P0.01;while the cell percentage in G_0/G_1 phase of cell cycle(67.72%、66.98%、66.31%、65.19%)decreased and the percentage in S phase(17.70% 18.27%、18.55%、19.11%)and G_2/M phase(14.58%、14.75%、15.14%、15.70%) increased.Conclusion Estrogen up-regulates the expression of KLK6 mRNA and protein and stimulates the H08910 cell proliferation.