Effect and mechanism of pueraria crude extract、pure puerarin on Lung Cancer H446 Cell proliferation

Objective To observe the effect of pure puerarin and pueraria crude extract on H446 cell proliferation, and explore its mechanism. Methods H446 cells grew in different concentrations of SP and CP, cell growth inhibition was detected by MTT assay.Cell cycle and apoptosis were detected by flow cytometry.Bcl-2 and Bax protein were detected by immunohistochemistry. Results The CP for 72 h 50% inhibitory concentration (IC50) was 435 μg/ml, for SP was 1 403 μg/ml、 435 μg/ml on H446 for 72 h,the apoptosis rate induced by CP and SP were respectively 14.71% and 2.61%, the percentages of G0/G1 phase cell numbers were respectively 79.20% and 72.20%, those of G2/M phase were respectively 8.94% and 10.50%, there was significant difference compared with control group(P0.05). Compared with the control group the Bcl-2 protein expression was lower, Bax expression is increased, P0.05. The expression proportion of Bax/Bcl-2 was higher than control group. Conclusions SP and CP inhibit H446 tumor cell proliferation. SP and CP can induce apoptosis, which may be related to block the cell cycle in the G0/G1 phase, up Bax Expression, lower Bcl-2 expression.