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2010•Unpublished venueRequires access

Role of TGF-β1 in the expression of Foxp3 and IL-10 on CD4~+CD25~(high) Treg in neonatal cord blood

Baiqing Li

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Abstract

To observe the expression of Foxp3 and IL-10 on CD4+CD25+ Treg induced by transforming growth factor-β1 (TGF-β1) in neonatal cord blood,cord blood mononuclear cells(CBMC) were stimulated with CD3 mAb and cultured in IL-2 containing medium with different concentrations of TGF-β1. The expressions of Foxp3 protein and Foxp3 gene mRNA in CD4+CD25+ Treg were detected by flow cytometry and RT-PCR assay,respectively,and the level of IL-10 in supernatant from cultured CBMC was detected by enzyme linked immunosorbent assay (ELISA). It was demonstrated that the expression of Foxp3 protein and mRNA in CD4+CD25+ Treg and IL-10 level in supernatant of cultured CBMC increased significantly in TGF-β1 treated group compared to control group. It is concluded that TGF-β1 up-regulates Foxp3 expression of the CD4+CD25+Treg and increases the secretion of IL-10 in cord blood in vitro.

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What this paper is about

To observe the expression of Foxp3 and IL-10 on CD4+CD25+ Treg induced by transforming growth factor-β1 (TGF-β1) in neonatal cord blood,cord blood mononuclear cells(CBMC) were stimulated with CD3 mAb and cultured in IL-2 containing medium with different concentrations of TGF-β1. The expressions of Foxp3 protein and Foxp3 gene mRNA in CD4+CD25+ Treg were detected by flow cytometry and RT-PCR assay,respectively,and the level of IL-10 in supernatant from cultured CBMC was detected by enzyme linked immunosorbent assay (ELISA). It was demonstrated that the expression of Foxp3 protein and mRNA in CD4+CD25+ Treg and IL-10 level in supernatant of cultured CBMC increased significantly in TGF-β1 treated group compared to control group. It is concluded that TGF-β1 up-regulates Foxp3 expression of the CD4+CD25+Treg and increases the secretion of IL-10 in cord blood in vitro.

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Available abstract

To observe the expression of Foxp3 and IL-10 on CD4+CD25+ Treg induced by transforming growth factor-β1 (TGF-β1) in neonatal cord blood,cord blood mononuclear cells(CBMC) were stimulated with CD3 mAb and cultured in IL-2 containing medium with different concentrations of TGF-β1. The expressions of Foxp3 protein and Foxp3 gene mRNA in CD4+CD25+ Treg were detected by flow cytometry and RT-PCR assay,respectively,and the level of IL-10 in supernatant from cultured CBMC was detected by enzyme linked immunosorbent assay (ELISA). It was demonstrated that the expression of Foxp3 protein and mRNA in CD4+CD25+ Treg and IL-10 level in supernatant of cultured CBMC increased significantly in TGF-β1 treated group compared to control group. It is concluded that TGF-β1 up-regulates Foxp3 expression of the CD4+CD25+Treg and increases the secretion of IL-10 in cord blood in vitro.

Key concepts: FOXP3, Cord blood, IL-2 receptor, Transforming growth factor, Peripheral blood mononuclear cell, Flow cytometry, Immunology, Transforming growth factor beta

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Role of TGF-β1 in the expression of Foxp3 and IL-10 on CD4~+CD25~(high) Treg in neonatal cord blood — Research Paper | ScholarLens