2008•Journal of Bengbu Medical CollegeRequires access
The dynamics study of proliferation of CD4~+CD25~+ Treg cells induced by transforming growth factor-β1 in neonatal cord blood
Baiqing Li
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Abstract
Objective:To explore proliferative activity of CD4+CD25+ Treg cells induced by transforming growth factor-β1(TGF-β1)in neonatal cord blood.Methods:Cord blood mononuclear cells were labeled with carboxyfluorescein diacetate,succinimidyl ester(CFSE),and then stimulated with TGF-β1,CD3mAb and cultured in 10% NBS-1640 medium in presence of IL-2.The dynamics of proliferation of CD4+CD25+ and CD8+ T cells on day 4,day 6,day 8 after culture was determined by flow cytometry.Results:Proliferative kinetics analysis showed proliferation index(PI)on day 6 and day 8 of culture with TGF-β1 in CD4+CD25+ Treg cells(11.52 and 23.04)were significantly higher than those in control cells(6.68 and 10.46)and those cultured with TGF-β1 in CD8+ T cells(4.23 and 5.43).On day 8 of culture with TGF-β1,proportion of the generations from 8th to 10th in CD4+CD25+ Treg(52.74%)was strikingly higher than those in control cells(36.26%)and those in CD8+ T cells cultured with TGF-β1(13.54%),while the proportion of the generations from 8th to 10th in control CD8+ T cells was 29.44%.Conclusions:Proliferative activity of CD4+CD25+ Treg cells induced by TGF-β1 in neonatal cord blood was obviously stronger than that of CD8+ T cells.